Hotspot

rpl32-trnL-UAG

SSC · 107574-108079 · 506 bp

Back to Diheteropogon

Pi 0.0318

MarkerSeek score 68.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 506
variable_sites: 27
pi: 0.0318
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAAGGATTGATTTGCGGCA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.2	58.0	80-859 bp	1.0	86.2
rpl32-trnL-UAG_p2	AGAAGGATTGATTTGCGGCA	GGATTCGAACCGAGATGCTT	45.0	50.0	58.2	58.1	80-862 bp	1.0	85.8
rpl32-trnL-UAG_p3	AGAAGGATTGATTTGCGGCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	80-832 bp	1.0	85.4
rpl32-trnL-UAG_p4	AGAAGGATTGATTTGCGGCA	GATCCGCACAAAAGAGTTGC	45.0	50.0	58.2	58.0	80-1411 bp	1.0	85.0
rpl32-trnL-UAG_p5	AGAAGGATTGATTTGCGGCA	ATGTCCCGAAGAAACCCAAC	45.0	50.0	58.2	58.1	80-1145 bp	1.0	84.9