Hotspot

trnN-GUU-rps15

SSC · 102359-103953 · 1595 bp

Back to Diheteropogon

Pi 0.00351
MarkerSeek score 54.20
Primer available yes
Label trnN-GUU-rps15

Feature Metadata

label_name
trnN-GUU-rps15
feature_type
igs
region
SSC
length_bp
1595
variable_sites
13
pi
0.00351
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rps15_p1 ACGACCAGTCAGTTAACAGC AATGTGAAGCAAGTCTCCGT 50.0 45.0 57.8 57.4 80-1735 bp 1.0 75.6
trnN-GUU-rps15_p2 AGGATTCGAACCTACGACCA AATGTGAAGCAAGTCTCCGT 50.0 45.0 58.2 57.4 80-1748 bp 1.0 75.5
trnN-GUU-rps15_p3 GGATTCGAACCTACGACCAG AATGTGAAGCAAGTCTCCGT 55.0 45.0 57.8 57.4 80-1747 bp 1.0 75.1
trnN-GUU-rps15_p4 CCCCAAGTAGGATTCGAACC AATGTGAAGCAAGTCTCCGT 55.0 45.0 57.7 57.4 80-1756 bp 1.0 74.2
trnN-GUU-rps15_p5 TTCGAACCTACGACCAGTCA AATGTGAAGCAAGTCTCCGT 50.0 45.0 58.4 57.4 80-1744 bp 1.0 73.6