Hotspot

psaA-pafI

LSC · 42513-43120 · 608 bp

Back to Diheteropogon

Pi 0.0163

MarkerSeek score 53.90

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 608
variable_sites: 22
pi: 0.0163
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	GGGGCCCTTAGCTATTGTTC	CTGAGCCGTATGAGGTAGGA	55.0	55.0	58.0	58.0	80-951 bp	1.0	86.3
psaA-pafI_p2	GGGGCCCTTAGCTATTGTTC	TCTGAGCCGTATGAGGTAGG	55.0	55.0	58.0	58.0	80-952 bp	1.0	86.3
psaA-pafI_p3	TAATGGAACCAACCGGCAAA	CTGAGCCGTATGAGGTAGGA	45.0	55.0	58.0	58.0	80-1364 bp	1.0	85.8
psaA-pafI_p4	TAATGGAACCAACCGGCAAA	TCTGAGCCGTATGAGGTAGG	45.0	55.0	58.0	58.0	80-1365 bp	1.0	85.8
psaA-pafI_p5	TAATGGAACCAACCGGCAAA	GAACAGGCCATTCTACAGGG	45.0	55.0	58.0	58.0	80-1294 bp	1.0	85.7