Hotspot

atpI-atpH

LSC · 32595-33417 · 823 bp

Back to Diheteropogon

Pi 0.00292

MarkerSeek score 54.60

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 5
pi: 0.00292
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	CAGTACCTTGACCAACTCCG	50.0	55.0	57.5	57.9	80-958 bp	1.0	76.6
atpI-atpH_p2	ATTTTTGCAACGTTAGCCGC	CAGTACCTTGACCAACTCCG	45.0	55.0	58.6	57.9	80-971 bp	1.0	76.1
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	80-959 bp	1.0	75.7
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	80-892 bp	1.0	75.7
atpI-atpH_p5	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	80-914 bp	1.0	75.6