Hotspot

rpoC2-rps2

LSC · 30570-30889 · 320 bp

Back to Diheteropogon

Pi 0.0184

MarkerSeek score 56.60

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 320
variable_sites: 12
pi: 0.0184
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	GAGACCCCGTTTACCCCTAT	ACCAAAATGAACTCCCGCTT	55.0	45.0	58.2	58.0	80-416 bp	1.0	85.1
rpoC2-rps2_p2	AGACCCCGTTTACCCCTATT	ACCAAAATGAACTCCCGCTT	50.0	45.0	57.7	58.0	80-415 bp	1.0	84.5
rpoC2-rps2_p3	CATCAGAGACCCCGTTTACC	ACCAAAATGAACTCCCGCTT	55.0	45.0	57.7	58.0	80-421 bp	1.0	84.2
rpoC2-rps2_p4	GAGACCCCGTTTACCCCTAT	TTTACGCTTTGCCGAGATGT	55.0	45.0	58.2	58.2	80-473 bp	1.0	83.0
rpoC2-rps2_p5	GAGACCCCGTTTACCCCTAT	GGGGACATTTTGGGATTCCA	55.0	50.0	58.2	57.8	80-451 bp	1.0	82.8