Hotspot

psbM-petN

LSC · 17985-18589 · 605 bp

Back to Diheteropogon

Pi 0.016

MarkerSeek score 70.50

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 605
variable_sites: 21
pi: 0.016
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGTTCATTCTAGTTCCTACTGCC	TAGTAAGTCTCGCTTGGGCT	43.5	50.0	57.9	58.2	80-789 bp	1.0	60.8
psbM-petN_p2	TGTTCATTCTAGTTCCTACTGCC	GTAGTATGGGGGAGGAGTGG	43.5	60.0	57.9	58.3	80-733 bp	1.0	59.9
psbM-petN_p3	TGTTCATTCTAGTTCCTACTGCC	TAGTATGGGGGAGGAGTGGA	43.5	55.0	57.9	58.4	80-732 bp	1.0	59.2
psbM-petN_p4	GTTCATTCTAGTTCCTACTGCCT	TAGTAAGTCTCGCTTGGGCT	43.5	50.0	57.7	58.2	80-788 bp	1.0	58.7
psbM-petN_p5	TGTTCATTCTAGTTCCTACTGCC	CGCTAGTAGTATGGGGGAGG	43.5	60.0	57.9	58.5	80-738 bp	1.0	58.6