Hotspot

trnT-GGU-trnE-UUC

LSC · 15974-16517 · 544 bp

Back to Diheteropogon

Pi 0.0188

MarkerSeek score 60.50

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 544
variable_sites: 22
pi: 0.0188
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	AGCCCTTTTAACTCAGTGGT	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.7	58.3	80-666 bp	1.0	74.3
trnT-GGU-trnE-UUC_p2	AGCCCTTTTAACTCAGTGGT	TGTCCTGAACCACTAGACGA	45.0	50.0	56.7	57.7	80-645 bp	1.0	74.3
trnT-GGU-trnE-UUC_p3	AGGCATAAGTCATCGGTTCA	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.6	58.3	80-629 bp	1.0	74.3
trnT-GGU-trnE-UUC_p4	AGGCATAAGTCATCGGTTCA	TGTCCTGAACCACTAGACGA	45.0	50.0	56.6	57.7	80-608 bp	1.0	74.3
trnT-GGU-trnE-UUC_p5	TGGTAAGGCATAAGTCATCGG	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	57.5	58.3	80-634 bp	1.0	73.5