Hotspot

trnS-GCU-psbD

LSC · 8356-9313 · 958 bp

Back to Diheteropogon

Pi 0.00522

MarkerSeek score 56.10

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 958
variable_sites: 11
pi: 0.00522
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1088-1119 bp	1.0	79.2
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	GAAACGGTCCCTTCGTAACC	45.0	55.0	58.0	58.6	1080-1111 bp	1.0	76.6
trnS-GCU-psbD_p3	GCTTTAGTCCACTCAGCCAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.6	58.2	1074-1105 bp	1.0	76.2
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	CTTCGTAACCAGTCGTCCAT	45.0	50.0	58.0	57.4	1070-1101 bp	1.0	76.0
trnS-GCU-psbD_p5	AGTCCACTCAGCCATCTCTC	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.5	58.2	1069-1100 bp	1.0	75.4