Hotspot

ccsA-ndhD

SSC · 108058-108265 · 208 bp

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Pi 0.0224

MarkerSeek score 67.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 208
variable_sites: 7
pi: 0.0224
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CCGCACTTGTAGCTTCGATA	CTCTCGCTATCCGTTGACAG	50.0	55.0	57.8	57.9	360-378 bp	1.0	82.8
ccsA-ndhD_p2	CCGCACTTGTAGCTTCGATA	TCTCTCGCTATCCGTTGACA	50.0	50.0	57.8	58.3	361-379 bp	1.0	81.7
ccsA-ndhD_p3	CCGCACTTGTAGCTTCGATA	GTTGACAGGGTAGAGGCTCT	50.0	55.0	57.8	58.4	348-366 bp	1.0	80.4
ccsA-ndhD_p4	CCGCACTTGTAGCTTCGATA	ATCCGTTGACAGGGTAGAGG	50.0	55.0	57.8	58.5	352-370 bp	1.0	79.8
ccsA-ndhD_p5	CCGCACTTGTAGCTTCGATA	GCTATCCGTTGACAGGGTAG	50.0	55.0	57.8	57.5	355-373 bp	1.0	79.7