Hotspot

rpl32-trnL-UAG

SSC · 106413-106937 · 525 bp

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Pi 0.0198

MarkerSeek score 67.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 525
variable_sites: 15
pi: 0.0198
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCCAAAAGGTTTTTCTGGGG	TCGAACCGAGATGCTTAAGC	50.0	50.0	57.7	58.1	636-648 bp	1.0	80.0
rpl32-trnL-UAG_p2	GCCAAAAGGTTTTTCTGGGG	AAGCACTGCTTCCTAAGAGC	50.0	50.0	57.7	57.9	620-632 bp	1.0	79.8
rpl32-trnL-UAG_p3	GCCAAAAGGTTTTTCTGGGG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.7	57.9	610-622 bp	1.0	79.7
rpl32-trnL-UAG_p4	GCCAAAAGGTTTTTCTGGGG	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.7	57.8	603-615 bp	1.0	79.2
rpl32-trnL-UAG_p5	GCCAAAAGGTTTTTCTGGGG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.7	57.8	608-620 bp	1.0	79.1