Hotspot

ndhF-rpl32

SSC · 105397-106220 · 824 bp

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Pi 0.0138

MarkerSeek score 68.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 824
variable_sites: 17
pi: 0.0138
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TCCAAAGATTTTTACGAATACGCT	55.0	33.3	58.1	57.3	935-942 bp	1.0	43.2
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	907-914 bp	1.0	43.0
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	908-915 bp	1.0	43.0
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	909-916 bp	1.0	43.0
ndhF-rpl32_p5	GGAAGTGGGAGAAGAGGGAT	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	910-917 bp	1.0	43.0