Hotspot

rpl14-rpl16

LSC · 77629-77731 · 103 bp

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Pi 0.0458
MarkerSeek score 71.40
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
103
variable_sites
7
pi
0.0458
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 AAGGGTCTGAGGTTGAATCA TAGCTGCCAGTAAAATGCCC 45.0 50.0 56.0 58.2 177-203 bp 1.0 66.3
rpl14-rpl16_p2 AAAGGGTCTGAGGTTGAATCA TAGCTGCCAGTAAAATGCCC 42.9 50.0 56.8 58.2 178-204 bp 1.0 64.5
rpl14-rpl16_p3 AGGGTCTGAGGTTGAATCAT TAGCTGCCAGTAAAATGCCC 45.0 50.0 55.8 58.2 176-202 bp 1.0 64.4
rpl14-rpl16_p4 AAGGGTCTGAGGTTGAATCAT TAGCTGCCAGTAAAATGCCC 42.9 50.0 56.6 58.2 177-203 bp 1.0 62.7
rpl14-rpl16_p5 AAAAGGGTCTGAGGTTGAATCA TAGCTGCCAGTAAAATGCCC 40.9 50.0 57.5 58.2 179-205 bp 1.0 62.2