Hotspot

atpB-rbcL

LSC · 54606-55360 · 755 bp

Back to Digitaria

Pi 0.00709

MarkerSeek score 61.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 755
variable_sites: 8
pi: 0.00709
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGATATCCAGCACAGGTCCA	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	58.1	58.2	928-940 bp	1.0	79.6
atpB-rbcL_p2	TGATATCCAGCACAGGTCCA	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	58.1	58.2	927-939 bp	1.0	79.6
atpB-rbcL_p3	TGATATCCAGCACAGGTCCA	CGTACTCCGGGGTGTAGTAA	50.0	55.0	58.1	58.2	931-943 bp	1.0	78.8
atpB-rbcL_p4	GTGATATCCAGCACAGGTCC	ACTCCGGGGTGTAGTAAGTC	55.0	55.0	57.8	58.2	929-941 bp	1.0	78.6
atpB-rbcL_p5	GTGATATCCAGCACAGGTCC	CTCCGGGGTGTAGTAAGTCA	55.0	55.0	57.8	58.2	928-940 bp	1.0	78.6