Hotspot

trnT-GGU-trnE-UUC

LSC · 14685-15146 · 462 bp

Back to Digitaria

Pi 0.0202

MarkerSeek score 67.30

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 462
variable_sites: 14
pi: 0.0202
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	AAAGGGCTTCTCACTTAGCG	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	57.9	58.3	581-586 bp	1.0	79.0
trnT-GGU-trnE-UUC_p2	AAAGGGCTTCTCACTTAGCG	TGTCCTGAACCACTAGACGA	50.0	50.0	57.9	57.7	560-565 bp	1.0	78.9
trnT-GGU-trnE-UUC_p3	CTTTGGTAAGACGGAAGGCA	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	57.8	58.3	550-555 bp	1.0	78.5
trnT-GGU-trnE-UUC_p4	TGCTTTGGTAAGACGGAAGG	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	57.8	58.3	552-557 bp	1.0	78.5
trnT-GGU-trnE-UUC_p5	CTTTGGTAAGACGGAAGGCA	TGTCCTGAACCACTAGACGA	50.0	50.0	57.8	57.7	529-534 bp	1.0	78.4