Hotspot

trnG-GCC-trnfM-CAU

LSC · 12254-12557 · 304 bp

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Pi 0.0307
MarkerSeek score 64.20
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
304
variable_sites
14
pi
0.0307
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 CGTAGCGAGCGTAGTTCAAT CGAGGCTCATAACCTTGAGG 50.0 55.0 58.2 57.8 445 bp 0.333333 50.1
trnG-GCC-trnfM-CAU_p2 CGTAGCGAGCGTAGTTCAAT CAGTTTGGTAGCTCACGAGG 50.0 55.0 58.2 58.3 460 bp 0.333333 49.6
trnG-GCC-trnfM-CAU_p3 CGTAGCGAGCGTAGTTCAAT CTACAGAGCGGAGTAGAGCA 50.0 55.0 58.2 58.3 478 bp 0.333333 49.3
trnG-GCC-trnfM-CAU_p4 CGTAGCGAGCGTAGTTCAAT TACAGAGCGGAGTAGAGCAG 50.0 55.0 58.2 58.3 477 bp 0.333333 49.3
trnG-GCC-trnfM-CAU_p5 CGTAGCGAGCGTAGTTCAAT GAGCGGAGTAGAGCAGTTTG 50.0 55.0 58.2 58.4 473 bp 0.333333 49.0