Hotspot

trnS-GCU-psbD

LSC · 7855-8837 · 983 bp

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Pi 0.0129

MarkerSeek score 57.50

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 983
variable_sites: 19
pi: 0.0129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	CTTCGTAACCAGTCGTCCAT	45.0	50.0	58.0	57.4	1097-1110 bp	1.0	75.9
trnS-GCU-psbD_p2	GCTTTAGTCCACTCAGCCAT	CTTCGTAACCAGTCGTCCAT	50.0	50.0	57.6	57.4	1083-1096 bp	1.0	72.9
trnS-GCU-psbD_p3	AGTCCACTCAGCCATCTCTC	CTTCGTAACCAGTCGTCCAT	55.0	50.0	58.5	57.4	1078-1091 bp	1.0	72.1
trnS-GCU-psbD_p4	GATTCGAACCCTCGGTACAA	CTTCGTAACCAGTCGTCCAT	50.0	50.0	57.3	57.4	1133-1146 bp	1.0	70.9
trnS-GCU-psbD_p5	TTAGCAATCCGCCGCTTTAG	CTTCGTAACCAGTCGTCCAT	50.0	50.0	58.7	57.4	1096-1109 bp	1.0	70.5