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ccsA-ndhD

SSC · 114786-115013 · 228 bp

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Pi 0.0482

MarkerSeek score 74.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 228
variable_sites: 21
pi: 0.0482
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGTTCATTTACATTTCATTGA	TTGGTGTGTACCCCGATTTC	27.3	50.0	52.3	57.8	327-330 bp	1.0	45.9
ccsA-ndhD_p2	TGGTTCATTTACATTTCATTGA	GGTGTGTACCCCGATTTCAT	27.3	50.0	52.3	57.6	325-328 bp	1.0	45.9
ccsA-ndhD_p3	ATGGTTCATTTACATTTCATTGA	TTGGTGTGTACCCCGATTTC	26.1	50.0	52.9	57.8	328-331 bp	1.0	45.8
ccsA-ndhD_p4	TGGTTCATTTACATTTCATTGA	CTCGGTATTGGTGTGTACCC	27.3	55.0	52.3	57.7	334-337 bp	1.0	45.7
ccsA-ndhD_p5	ATGGTTCATTTACATTTCATTGA	CTCGGTATTGGTGTGTACCC	26.1	55.0	52.9	57.7	335-338 bp	1.0	45.7