Hotspot

psaA-ycf3

LSC · 41505-42222 · 718 bp

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Pi 0.0146

MarkerSeek score 61.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 718
variable_sites: 22
pi: 0.0146
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTTTACTTCTGGCTCCGGC	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.5	58.0	850-876 bp	1.0	80.0
psaA-ycf3_p2	TTTTTACTTCTGGCTCCGGC	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.5	58.0	832-858 bp	1.0	80.0
psaA-ycf3_p3	TTTTTACTTCTGGCTCCGGC	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.5	57.8	779-805 bp	1.0	79.4
psaA-ycf3_p4	TTTTTACTTCTGGCTCCGGC	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.5	58.2	783-809 bp	1.0	79.1
psaA-ycf3_p5	TTTTTACTTCTGGCTCCGGC	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.5	58.3	846-872 bp	1.0	78.2