Hotspot

petN-psbM

LSC · 28848-29464 · 617 bp

Back to Digitalis

Pi 0.0149

MarkerSeek score 57.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 617
variable_sites: 20
pi: 0.0149
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCCCTTTTTGACTCTGCACC	AGTCCCGACGGATCCAATAT	50.0	50.0	58.0	58.0	1427-1456 bp	1.0	84.7
petN-psbM_p2	ACTCGTAGTGTGGGGAAGAA	AGTCCCGACGGATCCAATAT	50.0	50.0	58.0	58.0	1275-1296 bp	1.0	84.6
petN-psbM_p3	TCGTAGTGTGGGGAAGAAGT	AGTCCCGACGGATCCAATAT	50.0	50.0	58.0	58.0	1273-1294 bp	1.0	84.6
petN-psbM_p4	CGTAGTGTGGGGAAGAAGTG	AGTCCCGACGGATCCAATAT	55.0	50.0	57.9	58.0	1272-1293 bp	1.0	84.1
petN-psbM_p5	CCGGCAGGAAATCAGATTCA	AGTCCCGACGGATCCAATAT	50.0	50.0	58.0	58.0	1872-1904 bp	1.0	83.7