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trnH-GUG-psbA

LSC · 81-351 · 271 bp

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Pi 0.048

MarkerSeek score 77.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 271
variable_sites: 29
pi: 0.048
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CTATCGAAGCTCCAACAAATG	55.0	42.9	58.1	55.0	317-345 bp	1.0	56.3
trnH-GUG-psbA_p2	AATCCACTGCCTTGATCCAC	CTATCGAAGCTCCAACAAATG	50.0	42.9	57.9	55.0	330-358 bp	1.0	56.0
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CTATCGAAGCTCCAACAAATG	50.0	42.9	57.9	55.0	331-359 bp	1.0	55.9
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CTATCGAAGCTCCAACAAATG	50.0	42.9	57.9	55.0	332-360 bp	1.0	55.9
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TATCGAAGCTCCAACAAATG	55.0	40.0	58.1	53.7	316-344 bp	1.0	53.7