Hotspot

rps16

LSC · 5158-6278 · 1121 bp

Back to Diervilla

Pi 0

MarkerSeek score 59.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1121
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACGAATAGAACAAGAAAAAGGGA	TGTACCGACTGAACCAATGAC	34.8	47.6	56.1	58.0	1311-1317 bp	1.0	40.5
rps16_p2	ACGAATAGAACAAGAAAAAGGGA	GTACCGACTGAACCAATGACT	34.8	47.6	56.1	57.7	1310-1316 bp	1.0	40.5
rps16_p3	ACGAATAGAACAAGAAAAAGGGAA	TGTACCGACTGAACCAATGAC	33.3	47.6	56.8	58.0	1311-1317 bp	1.0	40.5
rps16_p4	ACGAATAGAACAAGAAAAAGGGA	TCAATTCCATACGGGTTCCA	34.8	45.0	56.1	56.5	1268-1274 bp	1.0	40.5
rps16_p5	ACGAATAGAACAAGAAAAAGGG	TGTACCGACTGAACCAATGAC	36.4	47.6	54.7	58.0	1311-1317 bp	1.0	40.5