Hotspot

ycf2-trnI-CAU

IRa · 157142-157641 · 500 bp

Back to Diervilla

Pi 0

MarkerSeek score 61.40

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 500
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	GGCCCAATGGAGTTCATAGG	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.0	58.0	852-879 bp	1.0	82.3
ycf2-trnI-CAU_p2	GGGCCCAATGGAGTTCATAG	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.0	58.0	853-880 bp	1.0	82.3
ycf2-trnI-CAU_p3	GGCCCAATGGAGTTCATAGG	ATTGGCATGCATCCAGTAGG	55.0	50.0	58.0	58.0	901-928 bp	1.0	82.2
ycf2-trnI-CAU_p4	GGGCCCAATGGAGTTCATAG	ATTGGCATGCATCCAGTAGG	55.0	50.0	58.0	58.0	902-929 bp	1.0	82.2
ycf2-trnI-CAU_p5	GGCCCAATGGAGTTCATAGG	ATGACCTCCTCCTCTATGCC	55.0	55.0	58.0	58.0	1554 bp	0.5	59.7