Hotspot

trnI-CAU-ycf2

IRb · 90072-90571 · 500 bp

Back to Diervilla

Pi 0

MarkerSeek score 61.40

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 500
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	GGCCCAATGGAGTTCATAGG	50.0	55.0	58.0	58.0	852-879 bp	1.0	82.3
trnI-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	GGGCCCAATGGAGTTCATAG	50.0	55.0	58.0	58.0	853-880 bp	1.0	82.3
trnI-CAU-ycf2_p3	ATTGGCATGCATCCAGTAGG	GGCCCAATGGAGTTCATAGG	50.0	55.0	58.0	58.0	901-928 bp	1.0	82.2
trnI-CAU-ycf2_p4	ATTGGCATGCATCCAGTAGG	GGGCCCAATGGAGTTCATAG	50.0	55.0	58.0	58.0	902-929 bp	1.0	82.2
trnI-CAU-ycf2_p5	ATGACCTCCTCCTCTATGCC	GGCCCAATGGAGTTCATAGG	55.0	55.0	58.0	58.0	1527-1554 bp	1.0	81.1