Hotspot

trnP-UGG-psaJ

LSC · 69977-70420 · 444 bp

Back to Diervilla

Pi 0

MarkerSeek score 55.70

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 444
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	AGTACTTAGTACCGGTGCCA	50.0	50.0	58.1	57.8	546-547 bp	1.0	77.6
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	AGTACTTAGTACCGGTGCCA	50.0	50.0	58.1	57.8	545-546 bp	1.0	77.6
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	AGTACTTAGTACCGGTGCCA	50.0	50.0	58.1	57.8	557-558 bp	1.0	77.5
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	AGTACTTAGTACCGGTGCCA	50.0	50.0	58.1	57.8	556-557 bp	1.0	77.5
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	AGTACTTAGTACCGGTGCCA	50.0	50.0	58.1	57.8	559-560 bp	1.0	77.5