Hotspot

ndhC-trnV-UAC

LSC · 52755-53836 · 1082 bp

Back to Diervilla

Pi 0.000924
MarkerSeek score 59.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1082
variable_sites
1
pi
0.000924
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT GCATGCTGGGTCTTTGAAAC 50.0 50.0 57.9 57.9 1277 bp 1.0 78.4
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AATGCATGCTGGGTCTTTGA 50.0 45.0 57.9 58.1 1280 bp 1.0 78.4
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT ATGCATGCTGGGTCTTTGAA 50.0 45.0 57.9 58.1 1279 bp 1.0 78.4
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1213 bp 1.0 77.4
ndhC-trnV-UAC_p5 CCTTTGCTAATCGGGGCTAA GCATGCTGGGTCTTTGAAAC 50.0 50.0 57.7 57.9 1289 bp 1.0 76.8