Hotspot

atpA-atpF

LSC · 12808-12862 · 55 bp

Back to Diervilla

Pi 0

MarkerSeek score 54.30

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 55
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ATGCCATCGCCTACTTGAAG	ATATTGACATGTTGGGGGCG	50.0	50.0	58.0	58.3	211-212 bp	1.0	77.6
atpA-atpF_p2	ATGCCATCGCCTACTTGAAG	GGGGGCGATGAACGAAATAA	50.0	50.0	58.0	58.3	198-199 bp	1.0	77.4
atpA-atpF_p3	ATGCCATCGCCTACTTGAAG	GGCGATGAACGAAATAACCG	50.0	50.0	58.0	57.6	195-196 bp	1.0	77.2
atpA-atpF_p4	CAATGCCATCGCCTACTTGA	ATATTGACATGTTGGGGGCG	50.0	50.0	58.3	58.3	213-214 bp	1.0	75.2
atpA-atpF_p5	CAATGCCATCGCCTACTTGA	GGGGGCGATGAACGAAATAA	50.0	50.0	58.3	58.3	200-201 bp	1.0	75.1