Hotspot

ycf1

SSC · 123569-128392 · 4824 bp

Back to Didymocarpus

Pi 0.0215

MarkerSeek score 58.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4824
variable_sites: 376
pi: 0.0215
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTCCTTTCCATTGACTCGGA	GCAGAATACCGTCACCCATT	47.6	50.0	57.7	58.0	204-233 bp	0.909091	77.4
ycf1_p2	TCTTCCTTTCCATTGACTCGG	GCAGAATACCGTCACCCATT	47.6	50.0	57.7	58.0	205-234 bp	0.909091	77.4
ycf1_p3	TTCCTTTCCATTGACTCGGA	GCAGAATACCGTCACCCATT	45.0	50.0	56.5	58.0	203-232 bp	0.909091	75.8
ycf1_p4	CTTCCTTTCCATTGACTCGGA	CGGAAGAAAGGGTGGAAAGT	47.6	50.0	57.7	57.7	171-182 bp	0.727273	67.1
ycf1_p5	CTTCCTTTCCATTGACTCGGA	ACGGAAGAAAGGGTGGAAAG	47.6	50.0	57.7	57.7	172-183 bp	0.727273	67.0