Hotspot

ndhF

SSC · 109894-112179 · 2286 bp

Back to Didymocarpus

Pi 0.02

MarkerSeek score 61.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2286
variable_sites: 162
pi: 0.02
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGATGCAACAACAAGACGTT	ACGTCGAATTTTGCAATCCG	45.0	45.0	57.6	57.8	2765-2825 bp	0.727273	67.4
ndhF_p2	TCGATGCAACAACAAGACGT	ACGTCGAATTTTGCAATCCG	45.0	45.0	58.4	57.8	2766-2826 bp	0.727273	67.1
ndhF_p3	CGATGCAACAACAAGACGTT	CGTCGAATTTTGCAATCCGA	45.0	45.0	57.6	57.5	2764-2824 bp	0.727273	65.2
ndhF_p4	TTCGATGCAACAACAAGACG	ACGTCGAATTTTGCAATCCG	45.0	45.0	57.3	57.8	2767-2827 bp	0.727273	65.2
ndhF_p5	TCGATGCAACAACAAGACGT	CGTCGAATTTTGCAATCCGA	45.0	45.0	58.4	57.5	2765-2825 bp	0.727273	64.9