Hotspot

ndhF-rpl32

SSC · 112180-112972 · 793 bp

Back to Didymocarpus

Pi 0.033

MarkerSeek score 75.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 793
variable_sites: 90
pi: 0.033
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCAAGAGGTCGTGTAAACCA	ACGATGCACAATACCCCTTC	50.0	50.0	58.1	58.0	1182-1398 bp	1.0	89.5
ndhF-rpl32_p2	AATAGAGCAGCACACAACGT	ACGATGCACAATACCCCTTC	45.0	50.0	57.8	58.0	1368-1584 bp	1.0	88.8
ndhF-rpl32_p3	GCAGCACACAACGTAACAAA	ACGATGCACAATACCCCTTC	45.0	50.0	57.8	58.0	1362-1578 bp	1.0	88.7
ndhF-rpl32_p4	GCAAGAGGTCGTGTAAACCA	TTCTCAAAGCGGCGAGAATT	50.0	45.0	58.1	58.2	1395-1610 bp	1.0	88.3
ndhF-rpl32_p5	AAACCAAAATCCCCTACCCG	ACGATGCACAATACCCCTTC	50.0	50.0	57.8	58.0	1247-1452 bp	0.727273	74.4