Hotspot

trnF-GAA-ndhJ

LSC · 48596-49231 · 636 bp

Back to Didymocarpus

Pi 0.0124

MarkerSeek score 66.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 636
variable_sites: 31
pi: 0.0124
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	55.0	45.0	58.0	58.2	721-819 bp	0.909091	81.9
trnF-GAA-ndhJ_p2	CTACATTGGGAATGGTCGGG	ATCATCCACGCTTGAAACGT	55.0	45.0	58.0	58.2	738-836 bp	0.909091	81.6
trnF-GAA-ndhJ_p3	TGCTACATTGGGAATGGTCG	ATCATCCACGCTTGAAACGT	50.0	45.0	58.0	58.2	740-838 bp	0.909091	81.6
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCTTGAAACGT	50.0	45.0	58.1	58.2	685-783 bp	0.909091	81.6
trnF-GAA-ndhJ_p5	GGATGGGATGCTACATTGGG	ATCATCCACGCTTGAAACGT	55.0	45.0	58.1	58.2	748-846 bp	0.909091	81.2