Hotspot

ycf3-trnS-GGA

LSC · 44665-45507 · 843 bp

Back to Didymocarpus

Pi 0.0142

MarkerSeek score 65.80

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 843
variable_sites: 40
pi: 0.0142
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCATTTATTCGCGATCTAGGC	CCCTCGGTAAACAAAAGCCT	47.6	50.0	57.0	58.1	702-953 bp	1.0	70.9
ycf3-trnS-GGA_p2	CCATTTATTCGCGATCTAGGC	ACGGAAAGAGAGGGATTCGA	47.6	50.0	57.0	58.2	723-974 bp	1.0	70.4
ycf3-trnS-GGA_p3	CCATTTATTCGCGATCTAGGC	TTCCAATGCTACGCCTTGAA	47.6	45.0	57.0	57.8	673-924 bp	1.0	70.2
ycf3-trnS-GGA_p4	CCATTTATTCGCGATCTAGGC	ACCCTCGGTAAACAAAAGCC	47.6	50.0	57.0	58.4	703-954 bp	1.0	68.6
ycf3-trnS-GGA_p5	TCCATTTATTCGCGATCTAGGC	CCCTCGGTAAACAAAAGCCT	45.5	50.0	58.4	58.1	703-954 bp	1.0	67.6