Hotspot

atpA-atpF

LSC · 12117-12192 · 76 bp

Back to Didymocarpus

Pi 0.0861

MarkerSeek score 72.90

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 76
variable_sites: 14
pi: 0.0861
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CACTTGAAGTACGGTACCGG	ACATGTTGGGAGCGATGAAA	55.0	45.0	58.0	57.8	194-259 bp	1.0	87.8
atpA-atpF_p2	CCACTTGAAGTACGGTACCG	ACATGTTGGGAGCGATGAAA	55.0	45.0	58.0	57.8	195-260 bp	1.0	87.8
atpA-atpF_p3	CAATACCATCGCCCACTTGA	ACATGTTGGGAGCGATGAAA	50.0	45.0	58.0	57.8	207-272 bp	1.0	87.3
atpA-atpF_p4	CACTTGAAGTACGGTACCGG	GACATGTTGGGAGCGATGAA	55.0	50.0	58.0	58.3	195-260 bp	1.0	87.3
atpA-atpF_p5	CCACTTGAAGTACGGTACCG	GACATGTTGGGAGCGATGAA	55.0	50.0	58.0	58.3	196-261 bp	1.0	87.3