Hotspot

ndhB

IRb · 95826-98039 · 2214 bp

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Pi 0.00136

MarkerSeek score 58.00

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: IRb
length_bp: 2214
variable_sites: 3
pi: 0.00136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	GCCCATTCCCATTTTGTTCG	TGGAGATTGGATGCAGTTACT	50.0	42.9	58.0	56.7	2341 bp	1.0	61.7
ndhB_p2	ATAACCCTAGCCCATTCCCA	TGGAGATTGGATGCAGTTACT	50.0	42.9	57.8	56.7	2350 bp	1.0	60.0
ndhB_p3	TAACCCTAGCCCATTCCCAT	TGGAGATTGGATGCAGTTACT	50.0	42.9	57.8	56.7	2349 bp	1.0	60.0
ndhB_p4	GCCCATTCCCATTTTGTTCG	TTGGAGATTGGATGCAGTTACT	50.0	40.9	58.0	57.4	2342 bp	1.0	59.4
ndhB_p5	CCCTAGCCCATTCCCATTTT	TGGAGATTGGATGCAGTTACT	50.0	42.9	57.5	56.7	2346 bp	1.0	58.0