Hotspot

rps7-ndhB

IRa · 143184-143505 · 322 bp

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Pi 0

MarkerSeek score 21.00

Primer available yes

Label rps7-ndhB

Feature Metadata

label_name: rps7-ndhB
feature_type: igs
region: IRa
length_bp: 322
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 21.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-ndhB_p1	ATGGCAGAGGCAAATAGAGC	TCATTCTGTACATGCCAGATCA	50.0	40.9	58.0	57.2	384 bp	1.0	57.8
rps7-ndhB_p2	TCCGAATTAGTGGATGCTGC	TCATTCTGTACATGCCAGATCA	50.0	40.9	58.0	57.2	450 bp	1.0	57.6
rps7-ndhB_p3	GGCAGAGGCAAATAGAGCTT	TCATTCTGTACATGCCAGATCA	50.0	40.9	58.0	57.2	382 bp	1.0	57.5
rps7-ndhB_p4	TTAGTGGATGCTGCCAAAGG	TCATTCTGTACATGCCAGATCA	50.0	40.9	58.2	57.2	444 bp	1.0	56.6
rps7-ndhB_p5	CGAATTAGTGGATGCTGCCA	TCATTCTGTACATGCCAGATCA	50.0	40.9	58.3	57.2	448 bp	1.0	55.3