Hotspot

trnL-UAA-trnF-GAA

LSC · 48668-49037 · 370 bp

Back to Dictamnus

Pi 0.00541

MarkerSeek score 46.70

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 370
variable_sites: 2
pi: 0.00541
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	TCAAGTCCCTCTATCCCCAC	TCTACCAGCTGAGCTATCCC	55.0	55.0	57.8	58.0	414 bp	1.0	79.0
trnL-UAA-trnF-GAA_p2	TCAAGTCCCTCTATCCCCAC	TTTGAACTGGTGACACGAGG	55.0	50.0	57.8	58.1	450 bp	1.0	78.5
trnL-UAA-trnF-GAA_p3	TCAAGTCCCTCTATCCCCAC	TCAGTCCTCTGCTCTACCAG	55.0	55.0	57.8	57.9	426 bp	1.0	77.9
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	426 bp	1.0	76.6
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	462 bp	1.0	76.1