Hotspot

psbZ-trnG-GCC

LSC · 36727-37278 · 552 bp

Back to Dictamnus

Pi 0.00373

MarkerSeek score 62.70

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 552
variable_sites: 2
pi: 0.00373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CGGATGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.4	57.8	684-700 bp	1.0	77.8
psbZ-trnG-GCC_p2	TTTGCTTCTCCGGATGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.5	57.8	694-710 bp	1.0	77.0
psbZ-trnG-GCC_p3	ATTTGCTTCTCCGGATGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.5	57.8	695-711 bp	1.0	76.6
psbZ-trnG-GCC_p4	CGGATGGTTGGTCGAGTAAC	ACATTACTTTACCCTGGCGG	55.0	50.0	58.4	57.6	723-739 bp	1.0	75.8
psbZ-trnG-GCC_p5	TTTGCTTCTCCGGATGGTTG	ACATTACTTTACCCTGGCGG	50.0	50.0	58.5	57.6	733-749 bp	1.0	74.9