Hotspot

matK-trnK-UUU

LSC · 3623-4339 · 717 bp

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Pi 0.00279

MarkerSeek score 60.10

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 717
variable_sites: 2
pi: 0.00279
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTCCCGAAAAAGAAGTGGG	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.7	58.2	820 bp	1.0	76.7
matK-trnK-UUU_p2	ACTCCCGAAAAAGAAGTGGG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.7	58.2	819 bp	1.0	76.7
matK-trnK-UUU_p3	CTCCCGAAAAAGAAGTGGGT	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.7	58.2	819 bp	1.0	76.7
matK-trnK-UUU_p4	CTCCCGAAAAAGAAGTGGGT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.7	58.2	818 bp	1.0	76.7
matK-trnK-UUU_p5	CTCCCGAAAAAGAAGTGGGT	GGGTTGCTAACTCAACGGTA	50.0	50.0	57.7	57.5	832 bp	1.0	74.3