Hotspot

petB

Genome · 8761-9976 · 1216 bp

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Pi 0.00493

MarkerSeek score 54.00

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: Genome
length_bp: 1216
variable_sites: 6
pi: 0.00493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TGTTAATACCTTCATTCAAATAGCT	TGGTAGTTTAATCGTGTAAGCA	28.0	36.4	54.8	55.5	1286-1292 bp	1.0	40.4
petB_p2	TGTTAATACCTTCATTCAAATAGCT	TGGTAGTTTAATCGTGTAAGCAA	28.0	34.8	54.8	56.2	1286-1292 bp	1.0	40.4
petB_p3	TTGTTAATACCTTCATTCAAATAGCT	TGGTAGTTTAATCGTGTAAGCA	26.9	36.4	55.5	55.5	1287-1293 bp	1.0	40.4
petB_p4	TGTTAATACCTTCATTCAAATAGC	TGGTAGTTTAATCGTGTAAGCA	29.2	36.4	53.4	55.5	1286-1292 bp	1.0	40.4
petB_p5	TGTTAATACCTTCATTCAAATAGCT	ATGGTAGTTTAATCGTGTAAGCA	28.0	34.8	54.8	56.0	1287-1293 bp	1.0	40.4