Hotspot

rps12-trnV-GAC

Genome · 85942-87316 · 1375 bp

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Pi 0.00364

MarkerSeek score 60.90

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: Genome
length_bp: 1375
variable_sites: 5
pi: 0.00364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	GCCACTTTACGTAAGGCAGA	GGCTCGAACTGATGACTTCC	50.0	55.0	57.9	58.4	1478-1479 bp	1.0	76.8
rps12-trnV-GAC_p2	GCCACTTTACGTAAGGCAGA	GCTCGAACTGATGACTTCCA	50.0	50.0	57.9	57.6	1477-1478 bp	1.0	76.7
rps12-trnV-GAC_p3	GCCACTTTACGTAAGGCAGA	CAGGCTCGAACTGATGACTT	50.0	50.0	57.9	57.6	1480-1481 bp	1.0	76.6
rps12-trnV-GAC_p4	GCCACTTTACGTAAGGCAGA	TCAGGCTCGAACTGATGACT	50.0	50.0	57.9	58.4	1481-1482 bp	1.0	76.0
rps12-trnV-GAC_p5	GCCACTTTACGTAAGGCAGA	CGAACTGATGACTTCCACCA	50.0	50.0	57.9	57.5	1474-1475 bp	1.0	76.0