Hotspot

trnL-CAA-ndhB

Genome · 82166-82358 · 193 bp

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Pi 0.0213
MarkerSeek score 67.20
Primer available yes
Label trnL-CAA-ndhB

Feature Metadata

label_name
trnL-CAA-ndhB
feature_type
igs
region
Genome
length_bp
193
variable_sites
4
pi
0.0213
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ndhB_p1 TCGAACCTCCACGCTTTAAA ACTTGTGTAATTGCTTCTATGT 45.0 31.8 57.5 53.7 353-358 bp 1.0 42.0
trnL-CAA-ndhB_p2 TTCGAACCTCCACGCTTTAA ACTTGTGTAATTGCTTCTATGT 45.0 31.8 57.5 53.7 354-359 bp 1.0 42.0
trnL-CAA-ndhB_p3 GATTCGAACCTCCACGCTTT ACTTGTGTAATTGCTTCTATGT 50.0 31.8 58.6 53.7 356-361 bp 1.0 42.0
trnL-CAA-ndhB_p4 TCGAACCTCCACGCTTTAAA ACTTGTGTAATTGCTTCTATGTT 45.0 30.4 57.5 54.6 353-358 bp 1.0 42.0
trnL-CAA-ndhB_p5 TTCGAACCTCCACGCTTTAA ACTTGTGTAATTGCTTCTATGTT 45.0 30.4 57.5 54.6 354-359 bp 1.0 42.0