Hotspot

trnK-UUU-chlB

Genome · 58528-59424 · 897 bp

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Pi 0.01

MarkerSeek score 60.60

Primer available yes

Label trnK-UUU-chlB

Feature Metadata

label_name: trnK-UUU-chlB
feature_type: igs
region: Genome
length_bp: 897
variable_sites: 9
pi: 0.01
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-chlB_p1	AACCTCTGTCAATGGCTTGG	TACGGAAAAATTTGCACGCC	50.0	45.0	58.1	57.9	1084-1085 bp	1.0	79.6
trnK-UUU-chlB_p2	AACCTCTGTCAATGGCTTGG	TGTATGCAGCCAAAGAAGCT	50.0	45.0	58.1	57.8	1031-1032 bp	1.0	78.5
trnK-UUU-chlB_p3	AGTTTCGCAAACCTCTGTCA	TACGGAAAAATTTGCACGCC	45.0	45.0	57.7	57.9	1093-1094 bp	1.0	77.7
trnK-UUU-chlB_p4	CCTCTGTCAATGGCTTGGAT	TACGGAAAAATTTGCACGCC	50.0	45.0	57.6	57.9	1082-1083 bp	1.0	76.9
trnK-UUU-chlB_p5	AACCTCTGTCAATGGCTTGG	AAATTTGCACGCCGAAATGA	50.0	40.0	58.1	57.6	1077-1078 bp	1.0	76.6