Hotspot

rps12-rpl20

Genome · 14746-15569 · 824 bp

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Pi 0.00365

MarkerSeek score 61.00

Primer available yes

Label rps12-rpl20

Feature Metadata

label_name: rps12-rpl20
feature_type: igs
region: Genome
length_bp: 824
variable_sites: 3
pi: 0.00365
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rpl20_p1	CTCAACGACGAGGAGTTTGT	CCTCGAAACCCCGATGTAAG	50.0	55.0	57.9	58.1	932-933 bp	1.0	78.7
rps12-rpl20_p2	CTCAACGACGAGGAGTTTGT	CTCGAAACCCCGATGTAAGG	50.0	55.0	57.9	58.1	931-932 bp	1.0	78.7
rps12-rpl20_p3	ACAAAATCTCCAGCCCTTCG	CCTCGAAACCCCGATGTAAG	50.0	55.0	58.2	58.1	960-961 bp	1.0	78.4
rps12-rpl20_p4	ACAAAATCTCCAGCCCTTCG	CTCGAAACCCCGATGTAAGG	50.0	55.0	58.2	58.1	959-960 bp	1.0	78.4
rps12-rpl20_p5	CTCAACGACGAGGAGTTTGT	AATTTCGAATGAGCCCCTCG	50.0	50.0	57.9	58.3	947-948 bp	1.0	76.6