Hotspot

atpB

LSC · 55529-57016 · 1488 bp

Back to Dicranostigma

Pi 0.481

MarkerSeek score 81.60

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1488
variable_sites: 629
pi: 0.481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	TATGGCATTCACCCACGAAG	TATTATTTGGACGCACCGCA	50.0	45.0	58.0	58.0	2493 bp	0.5	59.5
atpB_p2	TATGGCATTCACCCACGAAG	CCGCCCATCAGAGCTATTTT	50.0	50.0	58.0	58.0	2257 bp	0.5	59.5
atpB_p3	ACCAATCAGCTTGCTATCGG	TATTATTTGGACGCACCGCA	50.0	45.0	58.0	58.0	1984 bp	0.5	59.5
atpB_p4	ACCAATCAGCTTGCTATCGG	CCGCCCATCAGAGCTATTTT	50.0	50.0	58.0	58.0	1748 bp	0.5	59.5
atpB_p5	TATGGCATTCACCCACGAAG	TACGATGGGTCCCTTTTCCT	50.0	50.0	58.0	58.0	2778 bp	0.5	59.4