Hotspot

rps12-psbB

LSC · 74104-77178 · 3075 bp

Back to Dicranostigma

Pi 0.0909

MarkerSeek score 78.70

Primer available yes

Label rps12-psbB

Feature Metadata

label_name: rps12-psbB
feature_type: igs
region: LSC
length_bp: 3075
variable_sites: 262
pi: 0.0909
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-psbB_p1	TGGCTGGTTTAGATCGATCC	TTTTATCAAGCCCGAGGAGC	50.0	50.0	57.1	58.0	438 bp	0.5	52.4
rps12-psbB_p2	TGGCTGGTTTAGATCGATCC	CAAGTTCAAGACGCGTAGGA	50.0	50.0	57.1	57.9	333 bp	0.5	52.2
rps12-psbB_p3	TGGCTGGTTTAGATCGATCC	TCAAGTTCAAGACGCGTAGG	50.0	50.0	57.1	57.9	334 bp	0.5	52.2
rps12-psbB_p4	TGGCTGGTTTAGATCGATCC	GGTTTTTGAGGAGGGTGAGG	50.0	55.0	57.1	58.1	513 bp	0.5	52.0
rps12-psbB_p5	TGGCTGGTTTAGATCGATCC	GTTTTTGAGGAGGGTGAGGG	50.0	55.0	57.1	58.1	512 bp	0.5	52.0