Hotspot

trnF-GAA-ndhJ

LSC · 51038-51711 · 674 bp

Back to Dicranostigma

Pi 0.0426

MarkerSeek score 78.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 674
variable_sites: 28
pi: 0.0426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GTTGGATAGGATGGCCCTTG	50.0	55.0	58.1	58.0	779-806 bp	1.0	84.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	780-807 bp	1.0	83.4
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TCCACGCCTGAAACGTATTT	50.0	45.0	58.1	57.8	811-838 bp	1.0	82.6
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CATCCACGCCTGAAACGTAT	50.0	50.0	58.1	58.3	813-840 bp	1.0	81.2
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATT	50.0	45.0	58.1	57.6	812-839 bp	1.0	80.8