Hotspot

ndhI

SSC · 124922-125497 · 576 bp

Back to Dicranopteris

Pi 0.511

MarkerSeek score 80.80

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 576
variable_sites: 256
pi: 0.511
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TGTGAAAGGTTGCCATTCGA	AGACCTGTACAAGTTCCCGA	45.0	50.0	58.0	58.0	1648 bp	0.5	59.8
ndhI_p2	GCTGGTAGCGGGTTATCAAA	AGACCTGTACAAGTTCCCGA	50.0	50.0	58.0	58.0	1607 bp	0.5	59.7
ndhI_p3	TGTGAAAGGTTGCCATTCGA	CCTCACCTTCCTTTGCACTT	45.0	50.0	58.0	58.0	1160 bp	0.5	59.7
ndhI_p4	GCTGGTAGCGGGTTATCAAA	CCTCACCTTCCTTTGCACTT	50.0	50.0	58.0	58.0	1119 bp	0.5	59.6
ndhI_p5	GTGTGAAAGGTTGCCATTCG	AGACCTGTACAAGTTCCCGA	50.0	50.0	57.9	58.0	1649 bp	0.5	59.6