Hotspot

ndhH

SSC · 121497-122699 · 1203 bp

Back to Dicranopteris

Pi 0.471

MarkerSeek score 80.00

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1203
variable_sites: 467
pi: 0.471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GGATCCGCCGAATCTCAAAT	CGTACATAGGGGATGGGACT	50.0	55.0	58.1	58.0	2242 bp	0.5	59.0
ndhH_p2	GGATCCGCCGAATCTCAAAT	TAGGGGATGGGACTCGATTC	50.0	55.0	58.1	58.0	2236 bp	0.5	59.0
ndhH_p3	GGATCCGCCGAATCTCAAAT	CGCATAAAGCCAGGGGTATT	50.0	50.0	58.1	58.0	2081 bp	0.5	58.9
ndhH_p4	GGATCCGCCGAATCTCAAAT	CATAGGGGATGGGACTCGAT	50.0	55.0	58.1	58.1	2238 bp	0.5	58.6
ndhH_p5	GGATCCGCCGAATCTCAAAT	CTGCTCGGACATTCTCTCAC	50.0	55.0	58.1	58.1	2265 bp	0.5	58.4