Hotspot

ndhD

SSC · 127442-128950 · 1509 bp

Back to Dicranopteris

Pi 0.492

MarkerSeek score 81.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1509
variable_sites: 637
pi: 0.492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGGAGAATTTCAACGGCACA	CGAAAGAGACGTGGGCATTA	45.0	50.0	58.0	58.0	2384 bp	0.5	59.8
ndhD_p2	TGGAGAATTTCAACGGCACA	TTGCAGCATCTACGGGATTC	45.0	50.0	58.0	58.0	2291 bp	0.5	59.4
ndhD_p3	GAGCTTGTCCGACAGATGTT	CGAAAGAGACGTGGGCATTA	50.0	50.0	57.9	58.0	2317 bp	0.5	59.3
ndhD_p4	TGGAGAATTTCAACGGCACA	AGGTCAACCTGAGAGAGTCC	45.0	55.0	58.0	58.1	1961 bp	0.5	59.2
ndhD_p5	TGGAGAATTTCAACGGCACA	GTCAACCTGAGAGAGTCCCT	45.0	55.0	58.0	58.1	1959 bp	0.5	59.2