Hotspot

ccsA

SSC · 129233-130198 · 966 bp

Back to Dicranopteris

Pi 0.457

MarkerSeek score 79.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 358
pi: 0.457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTCAGGTTGACCTCATCAGC	CCCTACTCGGAATAGCAGGA	55.0	55.0	58.0	58.0	1793 bp	0.5	59.8
ccsA_p2	CTCAGGTTGACCTCATCAGC	TCCCCTACTCGGAATAGCAG	55.0	55.0	58.0	58.0	1795 bp	0.5	59.8
ccsA_p3	CTCAGGTTGACCTCATCAGC	GTAACAAGCCGCTATGGTGA	55.0	50.0	58.0	58.0	1547 bp	0.5	59.7
ccsA_p4	CTCTCCATTAACCGGTTGGG	CCCTACTCGGAATAGCAGGA	55.0	55.0	58.0	58.0	2274 bp	0.5	59.7
ccsA_p5	CTCTCCATTAACCGGTTGGG	TCCCCTACTCGGAATAGCAG	55.0	55.0	58.0	58.0	2276 bp	0.5	59.7