Hotspot

ndhG-psaC

SSC · 126294-127144 · 851 bp

Back to Dicranopteris

Pi 0.477

MarkerSeek score 82.40

Primer available yes

Label ndhG-psaC

Feature Metadata

label_name: ndhG-psaC
feature_type: igs
region: SSC
length_bp: 851
variable_sites: 363
pi: 0.477
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-psaC_p1	TCGGGAACTTGTACAGGTCT	CCCACATGAGGAAGAAGAGC	50.0	55.0	58.0	58.0	1869 bp	0.5	59.7
ndhG-psaC_p2	TCGGGAACTTGTACAGGTCT	GGGAGTGTTGGGAGCAATAG	50.0	55.0	58.0	58.0	1519 bp	0.5	59.6
ndhG-psaC_p3	TCGGGAACTTGTACAGGTCT	AAAATAGGCGCGTATTCCGT	50.0	45.0	58.0	58.1	1788 bp	0.5	59.5
ndhG-psaC_p4	TCGGGAACTTGTACAGGTCT	GACCCATACTGCGAGTTGTT	50.0	50.0	58.0	57.9	1299 bp	0.5	59.2
ndhG-psaC_p5	TCGGGAACTTGTACAGGTCT	TTACAACCATCCCGGGGTAT	50.0	50.0	58.0	58.1	1168 bp	0.5	59.1